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Depth mode provides enhanced depth of focus.
The depth of focus is inversely proportional to the angular aperture of the incident beam. In this mode, the intermediate lens works effectively as an "aperture changer" - its excitation changes will influence the beam so that the angular aperture of the incident beam can be set considerably lower than optimum. This means that the microscope does not work at the ultimate resolution, however in the range of lower and medium magnifications the difference in resolution cannot be seen. On the other hand, the difference in acquired depth of focus is clearly remarkable even at short working distances and often very useful, especially when investigating specimens with high topography.
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